Parkin immunoreactivity in the brain of human and non‐human primates: An immunohistochemical analysis in normal conditions and in Parkinsonian syndromes
Identifieur interne : 000204 ( France/Analysis ); précédent : 000203; suivant : 000205Parkin immunoreactivity in the brain of human and non‐human primates: An immunohistochemical analysis in normal conditions and in Parkinsonian syndromes
Auteurs : Martin Zarate-Lagunes [France] ; Wen-Jie Gu [France] ; Véronique Blanchard [France] ; Chantal Francois [France] ; Marie-Paule Muriel [France] ; Annick Mouatt-Prigent [France] ; Bruno Bonici [France] ; André Parent [France] ; Andreas Hartmann [France] ; Jérôme Yelnik [France] ; Georg A. Boehme [France] ; Laurent Pradier [France] ; Saliha Moussaoui [France] ; Baptiste Faucheux [France] ; Rita Raisman-Vozari [France] ; Yves Agid [France] ; Alexis Brice [France] ; Etienne C. Hirsch [France]Source :
- Journal of Comparative Neurology [ 0021-9967 ] ; 2001-04-02.
English descriptors
- KwdEn :
Abstract
The etiology of Parkinson's disease is unknown, but the gene involved in an autosomic recessive form of the disease with early onset has recently been identified. It codes for a protein with an unknown function called parkin. In the present study we produced a specific polyclonal antiserum against human parkin. Immunohistochemical analysis showed that parkin is expressed in neuronal perikarya and processes but also in glial and blood vessels in the primate brain (human and monkey). Electron microscopy indicated that parkin immunoreactivity is mostly located in large cytoplasmic vesicles and at the level of the endoplasmic reticulum. Parkin was expressed heterogeneously in various structures of the brain. It was detectable in the dopaminergic systems at the level of the perikarya in the mesencephalon but also in the striatum. However, parkin was also expressed by numerous nondopaminergic neurons. The staining intensity of parkin was particularly high in the hippocampal formation, the pallidal complex, the red nucleus, and the cerebellum. Comparison of control subjects with patients with Parkinson's disease and control animals with 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP)‐intoxicated animals revealed a loss of parkin‐immunoreactive neurons only in the substantia nigra pars compacta. Furthermore, the surviving dopaminergic neurons in the parkinsonian state continued to express parkin at a level similar to that observed in the control situation. These data indicate that parkin is a widely expressed protein. Thus, the degeneration of dopaminergic neurons in familial cases of Parkinson's disease with autosomal recessive transmission cannot be explained solely in terms of an alteration of this protein. J. Comp. Neurol. 432:184–196, 2001. © 2001 Wiley‐Liss, Inc.
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DOI: 10.1002/cne.1096
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Hirsch</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U 289, Hôpital de la Salpêtrière, 75013 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Comparative Neurology</title><title level="j" type="abbrev">J. Comp. Neurol.</title><idno type="ISSN">0021-9967</idno><idno type="eISSN">1096-9861</idno><imprint><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="2001-04-02">2001-04-02</date><biblScope unit="volume">432</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="184">184</biblScope><biblScope unit="page" to="196">196</biblScope></imprint><idno type="ISSN">0021-9967</idno></series><idno type="istex">25FAC305157A4B16A81C30141173AE2F51B97ADD</idno><idno type="DOI">10.1002/cne.1096</idno><idno type="ArticleID">CNE1096</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9967</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Parkinsonism</term><term>antibody</term><term>cell death</term><term>genetic</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The etiology of Parkinson's disease is unknown, but the gene involved in an autosomic recessive form of the disease with early onset has recently been identified. It codes for a protein with an unknown function called parkin. In the present study we produced a specific polyclonal antiserum against human parkin. Immunohistochemical analysis showed that parkin is expressed in neuronal perikarya and processes but also in glial and blood vessels in the primate brain (human and monkey). Electron microscopy indicated that parkin immunoreactivity is mostly located in large cytoplasmic vesicles and at the level of the endoplasmic reticulum. Parkin was expressed heterogeneously in various structures of the brain. It was detectable in the dopaminergic systems at the level of the perikarya in the mesencephalon but also in the striatum. However, parkin was also expressed by numerous nondopaminergic neurons. The staining intensity of parkin was particularly high in the hippocampal formation, the pallidal complex, the red nucleus, and the cerebellum. Comparison of control subjects with patients with Parkinson's disease and control animals with 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP)‐intoxicated animals revealed a loss of parkin‐immunoreactive neurons only in the substantia nigra pars compacta. Furthermore, the surviving dopaminergic neurons in the parkinsonian state continued to express parkin at a level similar to that observed in the control situation. These data indicate that parkin is a widely expressed protein. Thus, the degeneration of dopaminergic neurons in familial cases of Parkinson's disease with autosomal recessive transmission cannot be explained solely in terms of an alteration of this protein. J. Comp. Neurol. 432:184–196, 2001. © 2001 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li><li>Vitry‐Sur‐Seine</li></settlement><orgName><li>Hôpital de la Salpêtrière</li></orgName></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Zarate Agunes, Martin" sort="Zarate Agunes, Martin" uniqKey="Zarate Agunes M" first="Martin" last="Zarate-Lagunes">Martin Zarate-Lagunes</name></region><name sortKey="Agid, Yves" sort="Agid, Yves" uniqKey="Agid Y" first="Yves" last="Agid">Yves Agid</name><name sortKey="Blanchard, Veronique" sort="Blanchard, Veronique" uniqKey="Blanchard V" first="Véronique" last="Blanchard">Véronique Blanchard</name><name sortKey="Boehme, Georg A" sort="Boehme, Georg A" uniqKey="Boehme G" first="Georg A." last="Boehme">Georg A. Boehme</name><name sortKey="Bonici, Bruno" sort="Bonici, Bruno" uniqKey="Bonici B" first="Bruno" last="Bonici">Bruno Bonici</name><name sortKey="Brice, Alexis" sort="Brice, Alexis" uniqKey="Brice A" first="Alexis" last="Brice">Alexis Brice</name><name sortKey="Faucheux, Baptiste" sort="Faucheux, Baptiste" uniqKey="Faucheux B" first="Baptiste" last="Faucheux">Baptiste Faucheux</name><name sortKey="Francois, Chantal" sort="Francois, Chantal" uniqKey="Francois C" first="Chantal" last="Francois">Chantal Francois</name><name sortKey="Gu, Wen Ie" sort="Gu, Wen Ie" uniqKey="Gu W" first="Wen-Jie" last="Gu">Wen-Jie Gu</name><name sortKey="Hartmann, Andreas" sort="Hartmann, Andreas" uniqKey="Hartmann A" first="Andreas" last="Hartmann">Andreas Hartmann</name><name sortKey="Hirsch, Etienne C" sort="Hirsch, Etienne C" uniqKey="Hirsch E" first="Etienne C." last="Hirsch">Etienne C. Hirsch</name><name sortKey="Mouatt Rigent, Annick" sort="Mouatt Rigent, Annick" uniqKey="Mouatt Rigent A" first="Annick" last="Mouatt-Prigent">Annick Mouatt-Prigent</name><name sortKey="Moussaoui, Saliha" sort="Moussaoui, Saliha" uniqKey="Moussaoui S" first="Saliha" last="Moussaoui">Saliha Moussaoui</name><name sortKey="Muriel, Marie Aule" sort="Muriel, Marie Aule" uniqKey="Muriel M" first="Marie-Paule" last="Muriel">Marie-Paule Muriel</name><name sortKey="Parent, Andre" sort="Parent, Andre" uniqKey="Parent A" first="André" last="Parent">André Parent</name><name sortKey="Pradier, Laurent" sort="Pradier, Laurent" uniqKey="Pradier L" first="Laurent" last="Pradier">Laurent Pradier</name><name sortKey="Raisman Ozari, Rita" sort="Raisman Ozari, Rita" uniqKey="Raisman Ozari R" first="Rita" last="Raisman-Vozari">Rita Raisman-Vozari</name><name sortKey="Yelnik, Jerome" sort="Yelnik, Jerome" uniqKey="Yelnik J" first="Jérôme" last="Yelnik">Jérôme Yelnik</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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